Systemic Inflammation and the Breakdown of Intestinal Homeostasis Are Key Events in Chronic Spinal Cord Injury Patients

Our aim was to investigate the subset distribution and function of circulating monocytes, proinflammatory cytokine levels, gut barrier damage, and bacterial translocation in chronic spinal cord injury (SCI) patients. Thus, 56 SCI patients and 28 healthy donors were studied. The levels of circulating CD14^+highCD16⁻, CD14^+highCD16⁺, and CD14^+lowCD16⁺ monocytes, membrane TLR2, TLR4, and TLR9, phagocytic activity, ROS generation, and intracytoplasmic TNF-α, IL-1, IL-6, and IL-10 after lipopolysaccharide (LPS) stimulation were analyzed by polychromatic flow cytometry. Serum TNF-α, IL-1, IL-6 and IL-10 levels were measured by Luminex and LPS-binding protein (LBP), intestinal fatty acid-binding protein (I-FABP) and zonulin by ELISA. SCI patients had normal monocyte counts and subset distribution. CD14^+highCD16⁻ and CD14^+highCD16⁺ monocytes exhibited decreased TLR4, normal TLR2 and increased TLR9 expression. CD14^+highCD16⁻ monocytes had increased LPS-induced TNF-α but normal IL-1, IL-6, and IL-10 production. Monocytes exhibited defective phagocytosis but normal ROS production. Patients had enhanced serum TNF-α and IL-6 levels, normal IL-1 and IL-10 levels, and increased circulating LBP, I-FABP, and zonulin levels. Chronic SCI patients displayed impaired circulating monocyte function. These patients exhibited a systemic proinflammatory state characterized by enhanced serum TNF-α and IL-6 levels. These patients also had increased bacterial translocation and gut barrier damage.     

总变差模型在书画打印宣纸印刷质量预测中的应用

为了建立适用于书画打印宣纸印刷质量的预测模型,本研究测量了14种书画打印宣纸的粗糙度、白度、不透明度、定量、光泽度和针对宣纸特别设定的帘纹深浅以及帘纹疏密度等表面物理参量,并在相同条件下,使用喷墨打印设备输出并测量印品色度值,利用总变差模型构建去除帘纹色差的测定方法,得到与人眼视觉特征相符的色差。运用GRNN广义回归神经网络结合书画打印宣纸表面物理参量与宣纸去帘纹后的色差值,建立预测模型。结果表明,该模型能够在仅测量书画打印宣纸表面物理参量的情况下,便能较为准确地预测书画打印宣纸印刷质量,为书画打印宣纸印刷前的选纸工作提供指导依据。     

{X,Y}-Cyclacene Graphs with Next Nearest Neighbor Interactions

Eigensolutions of {X( = C,B,N),Y( = C,B,N)}-cyclacene graphs with next nearest neighbor (nnn) interactions have been obtained in analytical forms by adapting n-fold rotational symmetry followed by two-fold rotational symmetry (or a plane of symmetry). Expressions of eigensolution indicate the subspectral relationship among such cyclacenes with an even number of hexagonal rings e.g., eigenvalues of {X,Y}-di-cyclacene are found in the eigenspectra of all such even cyclacenes. Total π-electron energies and highest occupied molecular orbital and lowest unoccupied molecular orbital (HOMO–LUMO) gaps are calculated using the analytical expressions obtained and are found to vary negligibly with the variation of nnn interactions in such cyclacenes. Total π-electron energy is found to increase due to increase in restriction intensity of nnn interactions, whereas the HOMO–LUMO gap of polyacenecs having the even number of hexagonal rings and with one electron at each site (atom) decreases with increase in the restriction intensity since such systems contain degenerate half-filled HOMO (bonding or nonbonding) that are much more vulnerable for perturbations imposed through nnn interactions.     

碳脱氧在钢包顶渣改质中的应用

 为了进一步降低夹杂物缺陷并提高产品质量,基于碳脱氧进行了钢包顶渣改质的研究。冷轧产品的生产工艺为铁水预处理→转炉→RH精炼→连铸,为减少钢中夹杂物质量分数,需要进行钢包顶渣改质,同时降低钢包顶渣TFe质量分数。采用粒碳部分替代铝渣球的方法进行基于碳脱氧工艺的钢包顶渣改质,试验结果表明,顶渣改质效果良好,在顶渣TFe质量分数、中间包钢水游离氧明显降低的同时铸坯中Al₂O₃夹杂物得到优化;“30 kg粒渣+铝渣球”工艺降低生产成本5.16元/t(钢)。     

全尾砂和废石联合胶结充填体最佳配比及应用

针对红岭铅锌矿分级充填骨料来源不足、地表废石堆积污染环境的情况,提出将该矿选厂产生的全尾砂和地表废石作为充填骨料的联合胶结充填方案。分别测试了全尾砂和地表废石的物理化学性质,验证了碎石和全尾砂作为联合充填骨料的可行性。通过充填配比试验,分析了不同配比全尾砂废石充填体强度特性,得出了最佳充填配比。结果表明,充填试块的强度随灰砂比减小而减小,随养护龄期增加呈增大趋势,随充填料浆质量浓度增大而增大,最佳配比为: 胶结充填灰砂比1∶8、普通充填灰砂比1∶12,全尾砂∶废石配比4∶6,质量浓度78%。根据研究结果,在红岭铅锌矿进行了全尾砂废石充填技术工业应用,证实该技术可行。     

田赛距离测量中全站仪的设计与实现

本文提出了将智能全站仪这种精密测量仪器广泛应用于田赛项目测量中，并结合其他科技元素用于解决田赛项目中跳远、投掷等项目的测量及数据管理问题，实现实时、自动、准确地裁定和报告比赛成绩。     

不同含量低污染水对人工湿地中细菌的影响研究

探究水平流人工湿地(HFCW)系统处理低污染水过程中,相同COD/ρ(TN)下不同碳氮含量对细菌群落结构的影响。结果表明,进水为较高碳氮含量的HFCW(HF1)和进水为较低碳氮含量的HFCW(HF2)对COD和TN的去除效率具有一定的差异,HF1和HF2对COD的去除效率分别为48.26%和28.89%,对TN的去除率分别为79.06%和81.87%。HF1中细菌的丰富度和多样性均高于HF2,HF1中富集的优势细菌为Chloroflexaceae、Comamonadaceae和Rhodocyclaceae,均具有异养反硝化功能,HF2中富集的优势细菌为Xanthomonadaceae和Rhodocyclaceae,其中Xanthomonadaceae具有自养反硝化功能。COD、NH4^+-N和NO3^--N对HF1中细菌群落的影响大于对HF2中细菌群落的影响,HF1中COD对细菌群落的影响大于NH4^+-N和NO3^--N。     

Nonhydrolyzable Heptose Bis- and Monophosphate Analogues Modulate Pro-inflammatory TIFA-NF-κB Signaling

d -Glycero-d -manno-heptose-1β,7-bisphosphate (HBP) and d -glycero-d -manno-heptose-1β-phosphate (H1P) are bacterial metabolites that were recently shown to stimulate inflammatory responses in host cells through the activation of the TIFA-dependent NF-κB pathway. To better understand structure-based activity in relation to this process, a family of nonhydrolyzable phosphonate analogues of HBP and H1P was synthesized. The inflammation modulation by which these molecules induce the TIFA-NF-κB signal axis was evaluated in vivo at a low-nanomolar concentration (6 nM) and compared to that of the natural metabolites. Our data showed that three phosphonate analogues had similar stimulatory activity to HBP, whereas two phosphonates antagonized HBP-induced TIFA-NF-κB signaling. These results open new horizons for the design of pro-inflammatory and innate immune modulators that could be used as vaccine adjuvant.     

Fatal Attraction: How Bacterial Adhesins Affect Host Signaling and What We Can Learn from Them

The ability of bacterial species to colonize and infect host organisms is critically dependent upon their capacity to adhere to cellular surfaces of the host. Adherence to cell surfaces is known to be essential for the activation and delivery of certain virulence factors, but can also directly affect host cell signaling to aid bacterial spread and survival. In this review we will discuss the recent advances in the field of bacterial adhesion, how we are beginning to unravel the effects adhesins have on host cell signaling, and how these changes aid the bacteria in terms of their survival and evasion of immune responses. Finally, we will highlight how the exploitation of bacterial adhesins may provide new therapeutic avenues for the treatment of a wide range of bacterial infections.     

稠油微生物开采技术现状及进展

综述了用微生物方法开采稠油的技术现状与进展，论题如下。①概述。②基本方法：异源微生物采油，包括微生物吞吐和微生物驱；本源微生物采油压大港孔店油田的实例。③主要机理，包括产表面活性剂，降解稠油中重质组分及其他。④技术研究，包括机理性、可行性及经济效益研究，列举了国内外6个实例。⑤现场应用，包括国外1个、国内6个实例。⑥该技术的优势及问题。参22。